This means that water used for drinking, cooking, and bathing must be boiled first to kill bacteria. Boil advisories are issued if drinking water has unsafe numbers of bacteria. Natural waters that have unsafe levels of bacteria may be closed to recreation until they are safe again. Foods that have unsafe levels of bacteria are disposed of and protocols enacted to fix the bacterial overgrowth in the facility. If treated wastewater still has too many bacteria, the water will undergo further treatment prior to being released into the environment. What is the Determination of Bacterial Numbers Approach and What is it Used for?įigure 1: Professionals in government and industry measure the concentration of bacteria to maintain human safety and environmental health including: wastewater treatment (top left), food processing and food safety (top right), water recreation in oceans, lakes, ponds, rivers, and streams (bottom left), safety in swimming pools (bottom center), and safety of drinking water (bottom right).
Calculate CFU for samples and compare with safety standards to make conclusions about the safety of the samples.Successful conduct plate counts and absorbance readings.Use a standard line as well as a standard line equation to determine CFU of a sample from absorbance readings.Describe why a standard line correlating CFU and absorbance is important and how it can be used.Compare and contrast the standard plate count approach and absorbance measurements as it relates to determining cell density in a sample and the type of information these techniques provide about the cells.Tell what a spectrophotometer is and how it works.Explain how absorbance can be used as is a measure of sample turbidity and cell numbers.Explain how the standard plate count approach works.Give at least three real-world examples why determining bacterial numbers is an important technique.
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